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Product Info Summary
| SKU: | EKC2582 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2582
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The RIPK2 (Phospho-Ser176) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RIPK2 (Phospho-Ser176) protein expression profile in cells. The kit can be used for measuring the relative amounts of RIPK2 (Phospho-Ser176) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RIPK2 (Phospho-Ser176).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2582)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2582 is reactive to RIPK2 in Human, Mouse samples
Application Guarantee
EKC2582 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-RIPK2 (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-RIPK2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells treated with UV 15'
Protein Target Info & Infographic
Gene/Protein Information For RIPK2 (Source: Uniprot.Org, NCBI)
Gene Name
RIPK2
Full Name
Receptor-interacting serine/threonine-protein kinase 2
Weight
61.195kDa
Superfamily
protein kinase superfamily
Alternative Names
CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAKCCK; EC 2.7.11; EC 2.7.11.1; GIG30; growth-inhibiting gene 30; receptor interacting protein 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; receptor-interacting serine-threonine kinase 2; RICK; RICKGIG30; RIP2; RIP-2; RIP2CARD-carrying kinase; RI RIPK2 CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 receptor interacting serine/threonine kinase 2 receptor-interacting serine/threonine-protein kinase 2|CARD-carrying kinase|CARD-containing IL-1 beta ICE-kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|RIP-2|growth-inhibiting gene 30|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase|receptor-interacting protein 2|tyrosine-protein kinase RIPK2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RIPK2, check out the RIPK2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RIPK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA Kit (EKC2582)
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